USP28 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63015r-cy5 |
| Product Name | USP28 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human USP28 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57646 |
| Swiss Prot | Q96RU2 |
| Subcellular location | Nucleus |
| Synonyms | KIAA1515; Ubiquitin carboxyl terminal hydrolase 28; Ubiquitin thioesterase 28; UBP28. |
| Background | Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is USP28 Recombinant Antibody, Cy5 Conjugated (bsm-63015R-Cy5) validated for, and at what dilution?
bsm-63015R-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does USP28 Recombinant Antibody, Cy5 Conjugated (bsm-63015R-Cy5) react with?
Tested reactivity: Human.
How should bsm-63015R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise USP28 Recombinant Antibody, Cy5 Conjugated (bsm-63015R-Cy5)?
KLH conjugated synthetic peptide derived from human USP28
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-63015R-Cy5)?
Cy5 on bsm-63015R-Cy5: excitation / emission 625,650nm/670nm.