Phospho-VASP (Ser156) Recombinant Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-VASP (Ser156) Monoclonal Antibody, Unconjugated (bsm-63018R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-VASP (Ser156) Monoclonal Antibody, Unconjugated (bsm-63018R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-VASP (Ser156) Monoclonal Antibody, Unconjugated (bsm-63018R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-VASP (Ser156) Monoclonal Antibody, Unconjugated (bsm-63018R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Hela (H) cells were treated with or without Calyculin A (100ng\/mL) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-VASP (Ser156) monoclonal antibody, unconjugated (bsm-63018R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

Hela (H) cells were treated with or without Calyculin A (100ng\/mL) for 30 min, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-VASP (Ser156) monoclonal antibody, unconjugated (bsm-63018R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-63018r
Product Name	Phospho-VASP (Ser156) Recombinant Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human VASP around the phosphorylation site of S156
Modification Site	Ser156
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7408
Swiss Prot	P50552
Subcellular location	Cytoplasm
Synonyms	Vasodilator stimulated phosphoprotein; VASP.
Background	Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF