Phospho-VASP (Ser156) Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63018r-cy5-100uL |
| Product Name | Phospho-VASP (Ser156) Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VASP around the phosphorylation site of S156 |
| Modification Site | Ser156 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7408 |
| Swiss Prot | P50552 |
| Subcellular location | Cytoplasm |
| Synonyms | Vasodilator stimulated phosphoprotein; VASP. |
| Background | Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Phospho-VASP (Ser156) Recombinant Antibody, Cy5 Conjugated (bsm-63018R-Cy5) validated for, and at what dilution?
bsm-63018R-Cy5 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-VASP (Ser156) Recombinant Antibody, Cy5 Conjugated (bsm-63018R-Cy5) react with?
Tested reactivity: Human.
How should bsm-63018R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-VASP (Ser156) Recombinant Antibody, Cy5 Conjugated (bsm-63018R-Cy5)?
KLH conjugated synthetic peptide derived from human VASP around the phosphorylation site of S156
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-63018R-Cy5)?
Cy5 on bsm-63018R-Cy5: excitation / emission 625,650nm/670nm.