Phospho-VAV1 (Tyr174) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63020r-apc-cy5 |
| Product Name | Phospho-VAV1 (Tyr174) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VAV1 around the phosphorylation site of Y174 |
| Modification Site | Tyr174 |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7409 |
| Swiss Prot | P15498 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Oncogene vav; p95Vav;Proto-oncogene vav; VAV 1 oncogene; VAV;Vav proto oncogene; VAV1. |
| Background | Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-VAV1 (Tyr174) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-63020R-APC-Cy5) validated for, and at what dilution?
bsm-63020R-APC-Cy5 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-VAV1 (Tyr174) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-63020R-APC-Cy5) react with?
Tested reactivity: Human.
How should bsm-63020R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-VAV1 (Tyr174) Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-63020R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human VAV1 around the phosphorylation site of Y174
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-63020R-APC-Cy5)?
APC-Cy5 on bsm-63020R-APC-Cy5: excitation / emission 650nm/670nm.