S100A1 Monoclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63045r-bf350-100uL |
| Product Name | S100A1 Monoclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human S100A1 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6271 |
| Swiss Prot | P23297 |
| Subcellular location | Cytoplasm |
| Synonyms | S100; S-100 protein alpha subunit; S-100 protein alpha chain; Protein S100-A1; S100 calcium-binding protein A1; S100 alpha; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 Calcium Binding Protein A1; S100 calcium-binding protein A1; S100 protein alpha polypeptide; S100A; S10A1_HUMAN; S-100 α; S-100-α; S-100α. |
| Background | Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is S100A1 Monoclonal Antibody, AbBy Fluor-350 Conjugated (bsm-63045R-BF350) validated for, and at what dilution?
bsm-63045R-BF350 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does S100A1 Monoclonal Antibody, AbBy Fluor-350 Conjugated (bsm-63045R-BF350) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-63045R-BF350 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise S100A1 Monoclonal Antibody, AbBy Fluor-350 Conjugated (bsm-63045R-BF350)?
KLH conjugated synthetic peptide derived from human S100A1
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bsm-63045R-BF350)?
AbBy Fluor-350 on bsm-63045R-BF350: excitation / emission 346nm/442nm.