EZH2 Recombinant Antibody, PE-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63051r-pe-cy7-100uL |
| Product Name | EZH2 Recombinant Antibody, PE-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KMT6 |
| Clonality | Monoclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2146 |
| Swiss Prot | Q15910 |
| Subcellular location | Nucleus |
| Synonyms | Enhancer of zeste homolog 2; Enx1h; MGC9169; Enhancer of zeste 2; ENX-1; ENX 1; ENX1; EZH2; EZH 2; EZH2_HUMAN; Histone-lysine N-methyltransferase EZH2; KMT6A; Lysine N-methyltransferase 6; Enhancer of zeste homolog 2 (Drosophila); Enhancer of zeste, Drosophila, homolog 2; KMT 6; KMT6; KMT6A; WVS2. |
| Background | olycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is EZH2 Recombinant Antibody, PE-Cy7 Conjugated (bsm-63051R-PE-Cy7) validated for, and at what dilution?
bsm-63051R-PE-Cy7 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EZH2 Recombinant Antibody, PE-Cy7 Conjugated (bsm-63051R-PE-Cy7) react with?
Tested reactivity: Human.
How should bsm-63051R-PE-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EZH2 Recombinant Antibody, PE-Cy7 Conjugated (bsm-63051R-PE-Cy7)?
KLH conjugated synthetic peptide derived from human KMT6
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bsm-63051R-PE-Cy7)?
PE-Cy7 on bsm-63051R-PE-Cy7: excitation / emission 488nm/780nm.