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NPHS2 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Monoclonal Antibody, Unconjugated(bsm-63061R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Monoclonal Antibody, Unconjugated(bsm-63061R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Monoclonal Antibody, Unconjugated(bsm-63061R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Monoclonal Antibody, Unconjugated(bsm-63061R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with NPHS2 monoclonal antibody, unconjugated (bsm-63061R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with NPHS2 monoclonal antibody, unconjugated (bsm-63061R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NPHS2 Monoclonal Antibody, Unconjugated (bsm-63061R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NPHS2 Monoclonal Antibody, Unconjugated (bsm-63061R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
  • Rat
Overview
Catalog # bsm-63061r
Product Name NPHS2 Monoclonal Antibody
Applications
IHC-P IHC-F IF(IHC-P)
Reactivity Human, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human Podocin
Clonality Recombinant
Clone # 11B19
Isotype IgG
Concentration 1mg/ml
Purification purified by Protein A.
Storage Buffer 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7827
Swiss Prot Q9NP85
Subcellular location Cell membrane
Synonyms PDCN; SRN1; podocin; PODO_HUMAN; NPHS2; PODO_MOUSE; PODO_RAT; NPHS2 stomatin family member, podocin; nephrosis 2, idiopathic, steroid-resistant (podocin); NPHS2, podocin
Background Podocin is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
Application Dilution
IHC-P 1:100-500
IHC-F IHC-F1:100-500
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is NPHS2 Monoclonal Antibody (bsm-63061R) validated for, and at what dilution?

bsm-63061R is validated for IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NPHS2 Monoclonal Antibody (bsm-63061R) react with?

Tested reactivity: Human, Rat.

How should bsm-63061R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise NPHS2 Monoclonal Antibody (bsm-63061R)?

A synthesized peptide derived from human Podocin

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