NPHS2 Monoclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63061r-rbitc |
| Product Name | NPHS2 Monoclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7827 |
| Swiss Prot | Q9NP85 |
| Synonyms | nephrosis 2, idiopathic, steroid resistant; NPHS2 gene; PDCN; Podocin; SRN1; PODO_HUMAN |
| Background | Podocin is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. |
| Application Dilution | |
| IF(IHC-P) | 1:100-500 |
Frequently asked questions
Which applications is NPHS2 Monoclonal Antibody, RBITC Conjugated (bsm-63061R-RBITC) validated for, and at what dilution?
bsm-63061R-RBITC is validated for IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NPHS2 Monoclonal Antibody, RBITC Conjugated (bsm-63061R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-63061R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-63061R-RBITC)?
RBITC on bsm-63061R-RBITC: excitation / emission 570nm/595nm.