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PI3 kinase p150 Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated(bsm-63077R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with PI3 kinase p150 monoclonal antibody, unconjugated (bsm-63077R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PI3 kinase p150 monoclonal antibody, unconjugated (bsm-63077R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-F
  • IF(IHC-P)
  • IF(ICC)
  • IHC-P
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63077r-100uL
Product Name PI3 kinase p150 Monoclonal Antibody
Applications
WB IHC-F IF(IHC-P) IF(ICC) IHC-P FCM
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PI3 kinase p150
Clonality Monoclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 30849
Swiss Prot Q99570
Synonyms P150; Phosphoinositide 3 kinase regulatory subunit 4; PI3 kinase p150 subunit; PIK3R4; VPS15.
Application Dilution
WB 1:500-2000
IHC-F IHC-F1:100-500
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200
IHC-P IHC-P1:100-500
FCM FCM1:50-100

Frequently asked questions

Which applications is PI3 kinase p150 Monoclonal Antibody (bsm-63077R) validated for, and at what dilution?

bsm-63077R is validated for WB(WB) (1:500-2000); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200); IHC-P(IHC-P) (1:100-500); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PI3 kinase p150 Monoclonal Antibody (bsm-63077R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63077R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

What immunogen was used to raise PI3 kinase p150 Monoclonal Antibody (bsm-63077R)?

KLH conjugated synthetic peptide derived from human PI3 kinase p150

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