Histone H3.3(S22) Monoclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63099r-bf680-100uL |
| Product Name | Histone H3.3(S22) Monoclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human Histone H3.3 around the phosphorylation site of S22 |
| Clonality | Recombinant |
| Clone # | 5D12 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P84243 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | H3-3A | Histone H3.3 (p-S22); p-Histone H3.3; phospho-Histone H3.3; H3.3S22P; p-H3.3; BRYLIB1; H3.3A; H3F3; H3F3A; BRYLIB2; H3.3B; H3F3B; H3-3b; H33_HUMAN; H3-3A; H33_RAT; |
| Application Dilution | |
| WB | =1:500-2000 |
| IF | IF=1:50-200 |
Frequently asked questions
Which applications is Histone H3.3(S22) Monoclonal Antibody, AbBy Fluor-680 Conjugated (bsm-63099R-BF680) validated for, and at what dilution?
bsm-63099R-BF680 is validated for WB (WB=1:500-2000); IF (IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3.3(S22) Monoclonal Antibody, AbBy Fluor-680 Conjugated (bsm-63099R-BF680) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-63099R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3.3(S22) Monoclonal Antibody, AbBy Fluor-680 Conjugated (bsm-63099R-BF680)?
A synthesized peptide derived from human Histone H3.3 around the phosphorylation site of S22
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-63099R-BF680)?
AbBy Fluor-680 on bsm-63099R-BF680: excitation / emission 679nm/702nm.