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Vimentin Monoclonal Antibody, AbBy Fluor-594 Conjugated

Applications

  • WB
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63101r-bf594-100uL
Product Name Vimentin Monoclonal Antibody, AbBy Fluor-594 Conjugated
Applications
WB IF FLOW-CYT
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source A synthesized peptide derived from human Vimentin
Clonality Recombinant
Clone # 4E11
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Swiss Prot P08670
Subcellular location Cytoplasm
Synonyms VIME_HUMAN; VIM; VIME_MOUSE; VIME_RAT;
Application Dilution
WB =1:500-2000
IF IF=1:50-200
Flow-Cyt Flow-Cyt=1:50-100

Frequently asked questions

Which applications is Vimentin Monoclonal Antibody, AbBy Fluor-594 Conjugated (bsm-63101R-BF594) validated for, and at what dilution?

bsm-63101R-BF594 is validated for WB (WB=1:500-2000); IF (IF=1:50-200); Flow-Cyt (Flow-Cyt=1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Vimentin Monoclonal Antibody, AbBy Fluor-594 Conjugated (bsm-63101R-BF594) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63101R-BF594 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Vimentin Monoclonal Antibody, AbBy Fluor-594 Conjugated (bsm-63101R-BF594)?

A synthesized peptide derived from human Vimentin

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-63101R-BF594)?

AbBy Fluor-594 on bsm-63101R-BF594: excitation / emission 590nm/617nm.