Vimentin Monoclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63101r-pe-cy5.5-100uL |
| Product Name | Vimentin Monoclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human Vimentin |
| Clonality | Recombinant |
| Clone # | 4E11 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P08670 |
| Subcellular location | Cytoplasm |
| Synonyms | VIME_HUMAN; VIM; VIME_MOUSE; VIME_RAT; |
| Application Dilution | |
| WB | =1:500-2000 |
| Flow-Cyt | Flow-Cyt=1:50-100 |
Frequently asked questions
Which applications is Vimentin Monoclonal Antibody, PE-Cy5.5 Conjugated (BSM-63101R-PE-CY5-5) validated for, and at what dilution?
BSM-63101R-PE-CY5-5 is validated for WB (WB=1:500-2000); Flow-Cyt (Flow-Cyt=1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Vimentin Monoclonal Antibody, PE-Cy5.5 Conjugated (BSM-63101R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-63101R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Vimentin Monoclonal Antibody, PE-Cy5.5 Conjugated (BSM-63101R-PE-CY5-5)?
A synthesized peptide derived from human Vimentin
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BSM-63101R-PE-CY5-5)?
PE-Cy5.5 on BSM-63101R-PE-CY5-5: excitation / emission 488nm/694nm.