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BRAF Recombinant Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63118r-bf555-100uL
Product Name BRAF Recombinant Antibody, AbBy Fluor-555 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human B Raf
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 673
Swiss Prot P15056
Synonyms B-RAF; p94; NS7; B-Raf proto-oncogene serine/threonine-protein kinase;BRAF;BRAF1.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is BRAF Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-63118R-BF555) validated for, and at what dilution?

bsm-63118R-BF555 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BRAF Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-63118R-BF555) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63118R-BF555 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BRAF Recombinant Antibody, AbBy Fluor-555 Conjugated (bsm-63118R-BF555)?

KLH conjugated synthetic peptide derived from human B Raf

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bsm-63118R-BF555)?

AbBy Fluor-555 on bsm-63118R-BF555: excitation / emission 553nm/568nm.