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Mono-Methyl-Histone H3(R2) Monoclonal Antibody

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Mono-Methyl-Histone H3(R2)) monoclonal Antibody, unconjugated (bsm-63134R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Mono-Methyl-Histone H3(R2)) monoclonal Antibody, unconjugated (bsm-63134R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Mono-Methyl-Histone H3(R2) antibody (bsm-63134R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Mono-Methyl-Histone H3(R2) antibody (bsm-63134R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • ICC/IF

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-63134r
Product Name Mono-Methyl-Histone H3(R2) Monoclonal Antibody
Applications
WB ICC/IF
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human Histone H3.1 around the methylation site of R3
Clonality Recombinant
Clone # 4G10
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P68431
Subcellular location Nucleus
Synonyms H3C1 | Histone H3 (me1-R3); me-Histone H3; methyl-Histone H3; H3R3me1; me-H3;
Application Dilution
WB =1:1000-2000
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is Mono-Methyl-Histone H3(R2) Monoclonal Antibody (bsm-63134R) validated for, and at what dilution?

bsm-63134R is validated for WB (WB=1:1000-2000); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Mono-Methyl-Histone H3(R2) Monoclonal Antibody (bsm-63134R) react with?

Tested reactivity: Human, Mouse.

How should bsm-63134R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise Mono-Methyl-Histone H3(R2) Monoclonal Antibody (bsm-63134R)?

A synthesized peptide derived from human Histone H3.1 around the methylation site of R3

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