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Histone H2B (Acetyl K20) Monoclonal Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63153r-bf680-100uL
Product Name Histone H2B (Acetyl K20) Monoclonal Antibody, AbBy Fluor-680 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source A synthesized peptide derived from human Histone H2B around the acetylation site of K20
Clonality Recombinant
Clone # 8D1
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Swiss Prot P33778
Subcellular location Nucleus
Synonyms H2BC3 | Histone H2B (ac-K20); ac-Histone H2B; acetyl-Histone H2B; H2BK20Ac; ac-H2B; H2B.1; H2B/f; H2BFF; HIST1H2BB; H2B1B_HUMAN; H2BC3; H2B-clustered histone 3; Histone H2B.1; Histone H2B.f (H2B/f);
Application Dilution
WB =1:1000-5000
IF IF=1:50-200

Frequently asked questions

Which applications is Histone H2B (Acetyl K20) Monoclonal Antibody, AbBy Fluor-680 Conjugated (bsm-63153R-BF680) validated for, and at what dilution?

bsm-63153R-BF680 is validated for WB (WB=1:1000-5000); IF (IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Histone H2B (Acetyl K20) Monoclonal Antibody, AbBy Fluor-680 Conjugated (bsm-63153R-BF680) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63153R-BF680 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Histone H2B (Acetyl K20) Monoclonal Antibody, AbBy Fluor-680 Conjugated (bsm-63153R-BF680)?

A synthesized peptide derived from human Histone H2B around the acetylation site of K20

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-63153R-BF680)?

AbBy Fluor-680 on bsm-63153R-BF680: excitation / emission 679nm/702nm.