Histone H2B (Acetyl K20) Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63153r-cy3-100uL |
| Product Name | Histone H2B (Acetyl K20) Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human Histone H2B around the acetylation site of K20 |
| Clonality | Recombinant |
| Clone # | 8D1 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P33778 |
| Subcellular location | Nucleus |
| Synonyms | H2BC3 | Histone H2B (ac-K20); ac-Histone H2B; acetyl-Histone H2B; H2BK20Ac; ac-H2B; H2B.1; H2B/f; H2BFF; HIST1H2BB; H2B1B_HUMAN; H2BC3; H2B-clustered histone 3; Histone H2B.1; Histone H2B.f (H2B/f); |
| Application Dilution | |
| WB | =1:1000-5000 |
| IF | IF=1:50-200 |
| Flow-Cyt | Flow-Cyt=1:50-100 |
Frequently asked questions
Which applications is Histone H2B (Acetyl K20) Monoclonal Antibody, Cy3 Conjugated (bsm-63153R-Cy3) validated for, and at what dilution?
bsm-63153R-Cy3 is validated for WB (WB=1:1000-5000); IF (IF=1:50-200); Flow-Cyt (Flow-Cyt=1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H2B (Acetyl K20) Monoclonal Antibody, Cy3 Conjugated (bsm-63153R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-63153R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H2B (Acetyl K20) Monoclonal Antibody, Cy3 Conjugated (bsm-63153R-Cy3)?
A synthesized peptide derived from human Histone H2B around the acetylation site of K20
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-63153R-Cy3)?
Cy3 on bsm-63153R-Cy3: excitation / emission 512,550nm/570,615nm.