RAF1

$25 ug total protein per lane of various lysates (see on figure) probed with RAF1 monoclonal antibody, unconjugated (bsm-63185R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with RAF1 monoclonal antibody, unconjugated (bsm-63185R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-63185r-100ul
Product Name	RAF1
Applications	WB, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human c Raf
Clonality	Recombinant
Clone #	6B6
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5894
Swiss Prot	P04049
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	C-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1.
Application Dilution
WB	=1:500-2000
ICC/IF	ICC/IF=1:50-200