phospho-mTOR(S2448) Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-63190r-cy3-100uL |
| Product Name | phospho-mTOR(S2448) Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human mTOR around the phosphorylation site of S2448 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2475 |
| Swiss Prot | P42345 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | FRAP; FRAP1; FRAP2; RAFT1; Rapamycin target protein; kinase mTOR. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is phospho-mTOR(S2448) Recombinant Antibody, Cy3 Conjugated (bsm-63190R-Cy3) validated for, and at what dilution?
bsm-63190R-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-mTOR(S2448) Recombinant Antibody, Cy3 Conjugated (bsm-63190R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig.
How should bsm-63190R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-mTOR(S2448) Recombinant Antibody, Cy3 Conjugated (bsm-63190R-Cy3)?
A synthesized peptide derived from human mTOR around the phosphorylation site of S2448
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-63190R-Cy3)?
Cy3 on bsm-63190R-Cy3: excitation / emission 512,550nm/570,615nm.