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phospho-mTOR(S2448) Recombinant Antibody, HRP Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Pig
Overview
Catalog # bsm-63190r-hrp-100uL
Product Name phospho-mTOR(S2448) Recombinant Antibody, HRP Conjugated
Applications
WB IHC-P IHC-F
Reactivity Human, Mouse, Rat
Predicted Reactivity Pig
Specifications
Conjugation HRP
Host Rabbit
Source A synthesized peptide derived from human mTOR around the phosphorylation site of S2448
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2475
Swiss Prot P42345
Subcellular location Cytoplasm, Cell membrane
Synonyms FRAP; FRAP1; FRAP2; RAFT1; Rapamycin target protein; kinase mTOR.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:50-200
IHC-F IHC-F1:50-200

Frequently asked questions

Which applications is phospho-mTOR(S2448) Recombinant Antibody, HRP Conjugated (bsm-63190R-HRP) validated for, and at what dilution?

bsm-63190R-HRP is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-mTOR(S2448) Recombinant Antibody, HRP Conjugated (bsm-63190R-HRP) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig.

How should bsm-63190R-HRP be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-mTOR(S2448) Recombinant Antibody, HRP Conjugated (bsm-63190R-HRP)?

A synthesized peptide derived from human mTOR around the phosphorylation site of S2448