Histone H3(S28) Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-63239r-cy3-100uL |
| Product Name | Histone H3(S28) Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human Histone H3.1 around the phosphorylation site of S28 |
| Clonality | Recombinant |
| Clone # | 4A11 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus |
| Synonyms | H3C1 | Histone H3 (p-S29); p-Histone H3; phospho-Histone H3; H3S29P; p-H3; |
| Application Dilution | |
| WB | =1:500-2000 |
| IF | IF=1:50-200 |
Frequently asked questions
Which applications is Histone H3(S28) Monoclonal Antibody, Cy3 Conjugated (bsm-63239R-Cy3) validated for, and at what dilution?
bsm-63239R-Cy3 is validated for WB (WB=1:500-2000); IF (IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H3(S28) Monoclonal Antibody, Cy3 Conjugated (bsm-63239R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-63239R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H3(S28) Monoclonal Antibody, Cy3 Conjugated (bsm-63239R-Cy3)?
A synthesized peptide derived from human Histone H3.1 around the phosphorylation site of S28
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-63239R-Cy3)?
Cy3 on bsm-63239R-Cy3: excitation / emission 512,550nm/570,615nm.