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Histone H3(S28) Monoclonal Antibody, HRP Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-63239r-hrp-100uL
Product Name Histone H3(S28) Monoclonal Antibody, HRP Conjugated
Applications
WB IHC-P IHC-F
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation HRP
Host Rabbit
Source A synthesized peptide derived from human Histone H3.1 around the phosphorylation site of S28
Clonality Recombinant
Clone # 4A11
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Swiss Prot P68431
Subcellular location Nucleus
Synonyms H3C1 | Histone H3 (p-S29); p-Histone H3; phospho-Histone H3; H3S29P; p-H3;
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200

Frequently asked questions

Which applications is Histone H3(S28) Monoclonal Antibody, HRP Conjugated (bsm-63239R-HRP) validated for, and at what dilution?

bsm-63239R-HRP is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Histone H3(S28) Monoclonal Antibody, HRP Conjugated (bsm-63239R-HRP) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-63239R-HRP be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Histone H3(S28) Monoclonal Antibody, HRP Conjugated (bsm-63239R-HRP)?

A synthesized peptide derived from human Histone H3.1 around the phosphorylation site of S28