SUN1 Monoclonal Antibody

$The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SUN1 antibody (bsm-63252R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-SUN1 antibody (bsm-63252R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt

Reactivity

Human

Overview
Catalog #	bsm-63252r
Product Name	SUN1 Monoclonal Antibody
Applications	WB IHC-P IHC-F IF FLOW-CYT
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human SUN1
Clonality	Recombinant
Clone #	1G11
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms	UNC84A; 4632417G13Rik; 5730434D03Rik; mKIAA0810; SUN1_HUMAN; SUN1; Protein unc-84 homolog A; Sad1/unc-84 protein-like 1; KIAA0810; SUN1_MOUSE; Q5U2W0_RAT;
Application Dilution
WB	=1:1000-2000
IHC-P	IHC-P=1:100-200
IHC-F	IHC-F=1:100-200
IF	IF=1:100-200
Flow-Cyt	Flow-Cyt=1:50-100