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eIF4E Recombinant Antibody, FITC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63259r-fitc-100uL
Product Name eIF4E Recombinant Antibody, FITC Conjugated
Applications
WB FCM IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human eIF4E
Clonality Recombinant
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1977
Swiss Prot P06730
Subcellular location Cytoplasm, Nucleus
Synonyms CBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200

Frequently asked questions

Which applications is eIF4E Recombinant Antibody, FITC Conjugated (bsm-63259R-FITC) validated for, and at what dilution?

bsm-63259R-FITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does eIF4E Recombinant Antibody, FITC Conjugated (bsm-63259R-FITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63259R-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise eIF4E Recombinant Antibody, FITC Conjugated (bsm-63259R-FITC)?

KLH conjugated synthetic peptide derived from human eIF4E

What are the excitation and emission wavelengths of the FITC conjugate (bsm-63259R-FITC)?

FITC on bsm-63259R-FITC: excitation / emission 494nm/518nm.