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Mono-Methyl-Histone H3(K9) Monoclonal Antibody, Biotin Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F
  • IP

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63262r-biotin-100uL
Product Name Mono-Methyl-Histone H3(K9) Monoclonal Antibody, Biotin Conjugated
Applications
WB IHC-P IHC-F IP
Reactivity Human, Mouse, Rat
Specifications
Conjugation Biotin
Host Rabbit
Source A synthesized peptide derived from human Histone H3.1 around the methylation site of K10
Clonality Recombinant
Clone # 4E13
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Swiss Prot P68431
Subcellular location Nucleus
Synonyms H3C1 | Histone H3 (me1-K10); me-Histone H3; methyl-Histone H3; H3K10me1; me-H3;
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200
IP IP=1:20-50

Frequently asked questions

Which applications is Mono-Methyl-Histone H3(K9) Monoclonal Antibody, Biotin Conjugated (bsm-63262R-Biotin) validated for, and at what dilution?

bsm-63262R-Biotin is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IP (IP=1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Mono-Methyl-Histone H3(K9) Monoclonal Antibody, Biotin Conjugated (bsm-63262R-Biotin) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63262R-Biotin be stored?

Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Mono-Methyl-Histone H3(K9) Monoclonal Antibody, Biotin Conjugated (bsm-63262R-Biotin)?

A synthesized peptide derived from human Histone H3.1 around the methylation site of K10

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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