DiMethyl-Histone H3(K4) Monoclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63264r-cy5.5-100uL |
| Product Name | DiMethyl-Histone H3(K4) Monoclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | A synthesized peptide derived from human Histone H3.1 around the methylation site of K4 |
| Clonality | Recombinant |
| Clone # | 2C11 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus |
| Synonyms | H3C1 | Histone H3 (me2-K5); me2-Histone H3; di-methyl-Histone H3; H3K5me2; me2-H3; |
| Application Dilution | |
| WB | =1:500-2000 |
| IF | IF=1:50-200 |
Frequently asked questions
Which applications is DiMethyl-Histone H3(K4) Monoclonal Antibody, Cy5.5 Conjugated (BSM-63264R-CY5-5) validated for, and at what dilution?
BSM-63264R-CY5-5 is validated for WB (WB=1:500-2000); IF (IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DiMethyl-Histone H3(K4) Monoclonal Antibody, Cy5.5 Conjugated (BSM-63264R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-63264R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DiMethyl-Histone H3(K4) Monoclonal Antibody, Cy5.5 Conjugated (BSM-63264R-CY5-5)?
A synthesized peptide derived from human Histone H3.1 around the methylation site of K4
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-63264R-CY5-5)?
Cy5.5 on BSM-63264R-CY5-5: excitation / emission 675nm/694nm.