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DiMethyl-Histone H3(K4) Monoclonal Antibody, PE Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63264r-pe
Product Name DiMethyl-Histone H3(K4) Monoclonal Antibody, PE Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation PE
Host Rabbit
Source A synthesized peptide derived from human Histone H3.1 around the methylation site of K4
Clonality Recombinant
Clone # 2C11
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Swiss Prot P68431
Subcellular location Nucleus
Synonyms H3C1 | Histone H3 (me2-K5); me2-Histone H3; di-methyl-Histone H3; H3K5me2; me2-H3;
Application Dilution
WB =1:500-2000
IF IF=1:50-200

Frequently asked questions

Which applications is DiMethyl-Histone H3(K4) Monoclonal Antibody, PE Conjugated (bsm-63264R-PE) validated for, and at what dilution?

bsm-63264R-PE is validated for WB (WB=1:500-2000); IF (IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does DiMethyl-Histone H3(K4) Monoclonal Antibody, PE Conjugated (bsm-63264R-PE) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63264R-PE be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise DiMethyl-Histone H3(K4) Monoclonal Antibody, PE Conjugated (bsm-63264R-PE)?

A synthesized peptide derived from human Histone H3.1 around the methylation site of K4

What are the excitation and emission wavelengths of the PE conjugate (bsm-63264R-PE)?

PE on bsm-63264R-PE: excitation / emission 496,564nm/578nm.