H3C1 | Histone H3 (ac-K36) Recombinant Antibody

$Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells (treated with 5 mM sodium butyrate for 24 hours) per IP, with 5 \u03bcg of bsm-63381R antibody or 5 \u03bcg of normal rabbit IgG(negative control bs-0295P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, LDHA and RPL30 exon 3 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.$

Chromatin immunoprecipitation was performed using cross-linked chromatin from 5\u00d710\u2076 HeLa cells (treated with 5 mM sodium butyrate for 24 hours) per IP, with 5 \u03bcg of bsm-63381R antibody or 5 \u03bcg of normal rabbit IgG(negative control bs-0295P) , and 20 \u03bcL of Protein A\/G MagBeads per IP. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH CDS, LDHA and RPL30 exon 3 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.

Applications

WB
IHC-F
ChIP
IHC-P
ICC/IF

Predicted Reactivity

Human
Mouse
Rat
Pig

Overview
Catalog #	bsm-63381r-100ul
Product Name	H3C1 \| Histone H3 (ac-K36) Recombinant Antibody
Applications	WB IHC-F CHIP IHC-P ICC/IF
Predicted Reactivity	Human, Mouse, Rat, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8968
Swiss Prot	Q71DI3
Subcellular location	Nucleus
Application Dilution
WB	1:500-2000
IHC-F	1:50-200
ChIP	5μg/IP
IHC-P	1:50-200
ICC/IF	1:50-100