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PKA C-alpha/beta/gamma (p-T197) Recombinant Antibody

Jurkat (H) serum starve for 24 h then 20% FBS 20 h , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-PKA C-alpha & beta & gamma (Thr197) monoclonal antibody, unconjugated (bsm-63522R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Jurkat (H) serum starve for 24 h then 20% FBS 20 h , 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with Phospho-PKA C-alpha & beta & gamma (Thr197) monoclonal antibody, unconjugated (bsm-63522R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-PKA C-alpha & beta & gamma (Thr197) monoclonal antibody, unconjugated (bsm-63522R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-PKA C-alpha & beta & gamma (Thr197) monoclonal antibody, unconjugated (bsm-63522R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T197) Monoclonal Antibody, Unconjugated (bsm-63522R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IP
  • IF
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63522r-100ul
Product Name PKA C-alpha/beta/gamma (p-T197) Recombinant Antibody
Applications
WB ELISA IHC-F IP IF IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Clonality Recombinant
Clone # 10G6
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5566
Swiss Prot P17612
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms cAMP-dependent protein kinase catalytic subunit alpha/beta;PRKACA;PKACA;cAMP-dependent protein kinase catalytic subunit alpha;PKA C-alpha;PRKACB;cAMP-dependent protein kinase catalytic subunit beta;PKA C-beta;PRKACG;cAMP-dependent protein kinase catalytic subunit gamma;PKA C-gamma
Application Dilution
WB 1:500-2000
ELISA 1:5000-10000
IHC-F 1:50-200
IP 1:50-200
IF 1:50-200
IHC-P 1:50-200

Frequently asked questions

Which applications is PKA C-alpha/beta/gamma (p-T197) Recombinant Antibody (bsm-63522R) validated for, and at what dilution?

bsm-63522R is validated for WB (1:500-2000); ELISA (1:5000-10000); IHC-F (1:50-200); IP (1:50-200); IF (1:50-200); IHC-P (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PKA C-alpha/beta/gamma (p-T197) Recombinant Antibody (bsm-63522R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63522R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

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