Glyceraldehyde 3-Phosphate Dehydrogenase Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70126M-100uL |
| Product Name | Glyceraldehyde 3-Phosphate Dehydrogenase Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~38 kDa Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein. |
| Reactivity | Human, Mouse, Rat, Bovine |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Purified porcine GAPDH. |
| Clonality | Monoclonal |
| Clone # | 1D4 |
| Isotype | IgM |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 50% glycerol and 5 mM NaN3 |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 26330 |
| Swiss Prot | P00355 |
| Synonyms | 38 kDa BFA-dependent ADP-ribosylation substrate antibody, aging associated gene 9 protein antibody, Aging-associated gene 9 protein antibody, BARS-38 antibody, cb609 antibody, EC 1.2.1.12 antibody, Epididymis secretory sperm binding protein Li 162eP antibody, G3P_HUMAN antibody, G3PD antibody, G3PDH antibody, GAPD antibody, GAPDH antibody, Glyceraldehyde 3 phosphate dehydrogenase antibody, Glyceraldehyde-3-phosphate dehydrogenase antibody, HEL-S-162eP antibody, KNC-NDS6 antibody, MGC102544 antibody, MGC102546 antibody, MGC103190 antibody, MGC103191 antibody, MGC105239 antibody, MGC127711 antibody, MGC88685 antibody, OCAS, p38 component antibody, OCT1 coactivator in S phase, 38-KD component antibody, peptidyl cysteine S nitrosylase GAPDH antibody, Peptidyl-cysteine S-nitrosylase GAPDH antibody, wu:fb33a10 antibody |
| Background | Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | 1:50-200 |
Frequently asked questions
Which applications is Glyceraldehyde 3-Phosphate Dehydrogenase Antibody (bsm-70126M) validated for, and at what dilution?
bsm-70126M is validated for WB (1:300-5000); IHC (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Glyceraldehyde 3-Phosphate Dehydrogenase Antibody (bsm-70126M) react with?
Tested reactivity: Human, Mouse, Rat, Bovine.
How should bsm-70126M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 50% glycerol and 5 mM NaN3
What immunogen was used to raise Glyceraldehyde 3-Phosphate Dehydrogenase Antibody (bsm-70126M)?
Purified porcine GAPDH.