Neurofilament NF-H Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70169M |
| Product Name | Neurofilament NF-H Antibody |
| Applications |
WB
IHC
|
| Specificity | Specific for endogenous levels of the ~200 kDa Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections. |
| Reactivity | Human, Mouse, Rat, Chicken, All Known Mammal |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Purified bovine NF-H |
| Clonality | Monoclonal |
| Clone # | NAP4 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 10 mM NaN3. |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Gene ID | 4744 |
| Swiss Prot | P12036 |
| Synonyms | 200 kDa neurofilament protein antibody, CMT2CC antibody, Nefh antibody, Neurofilament heavy polypeptide 200kDa antibody, Neurofilament heavy polypeptide antibody, Neurofilament triplet H protein antibody, NF H antibody, NF-H antibody, NFH antibody, NFH_HUMAN antibody |
| Background | Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3). |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC | |
Frequently asked questions
Which applications is Neurofilament NF-H Antibody (bsm-70169M) validated for, and at what dilution?
bsm-70169M is validated for WB (1:300-5000); IHC; ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Neurofilament NF-H Antibody (bsm-70169M) react with?
Tested reactivity: Human, Mouse, Rat, Chicken, All Known Mammal.
How should bsm-70169M be stored?
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy Storage buffer: PBS + 10 mM NaN3.
What immunogen was used to raise Neurofilament NF-H Antibody (bsm-70169M)?
Purified bovine NF-H