Thyroid Hormone Receptor, _2-Isotype Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-70287M |
| Product Name | Thyroid Hormone Receptor, _2-Isotype Antibody |
| Applications |
WB
|
| Specificity | Specific for endogenous levels of the ~58 kDa TR-α2 protein. |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Bovine, Non-Human Primate |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Synthetic peptide corresponding to amino acid residues from the C-terminal region of human thyroid hormone receptor, α2-isotype, conjugated to keyhole limpet hemocyanin (KLH). |
| Clonality | Monoclonal |
| Clone # | 1330 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Gene ID | 7067 |
| Swiss Prot | P10827 |
| Synonyms | c-erbA-1 antibody, C-erbA-alpha antibody, EAR-7 antibody, ERBA 1 antibody, ERBA antibody, ERBA related 7 antibody, Nuclear receptor subfamily 1 group A member 1 antibody, THA_HUMAN antibody, THRA antibody, Thra1 antibody, Thra2 antibody, Thyroid hormone receptor alpha 1 antibody, Thyroid hormone receptor alpha 2 antibody, Thyroid hormone receptor alpha antibody, TR alpha 1 antibody, TR alpha 2 antibody, Tra1 antibody, Triiodothyronine receptor antibody, V-erbA-related protein 7 antibody |
| Background | Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory (Ambrogini et al., 2005; Chan and Kilby, 2000). Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors, TRα1, TRα2, TRβ1 and TRβ2. TRα1, TRβ1 and TRβ2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain (Constantinou et al., 2005). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Thyroid Hormone Receptor, _2-Isotype Antibody (bsm-70287M) validated for, and at what dilution?
bsm-70287M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Thyroid Hormone Receptor, _2-Isotype Antibody (bsm-70287M) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Bovine, Non-Human Primate.
How should bsm-70287M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
What immunogen was used to raise Thyroid Hormone Receptor, _2-Isotype Antibody (bsm-70287M)?
Synthetic peptide corresponding to amino acid residues from the C-terminal region of human thyroid hormone receptor, α2-isotype, conjugated to keyhole limpet hemocyanin (KLH).