P-Cadherin (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70369M |
| Product Name | P-Cadherin (N-terminal region) Antibody |
| Applications |
WB
|
| Specificity | The P-cadherin antibody detects a 120 kDa* protein corresponding to endogenous P-Cadherin expressed in human A431 cells. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M596) was generated from a recombinant protein containing amino acids in the N-terminal region of human P-Cadherin. This sequence is highly conserved in mouse and rat P-cadherin. |
| Clonality | Monoclonal |
| Clone # | M596 |
| Isotype | IgG1 |
| Purification | Purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P22223 |
| Synonyms | CDH3, Cadherin 3, CDHP, Placental Cadherin, P-Cadherin |
| Background | Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. P-Cadherin (Cadherin-3) is localized in placenta while E-Cadherin (Cadherin-1) and N-Cadherin (Cadherin-2) are found in epithelial and neural tissues, respectively. P-Cadherin is expressed in normal epithelial cells and some cancer cells, and its sequence contains 5 cadherin domains in the extracellular region. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is P-Cadherin (N-terminal region) Antibody (bsm-70369M) validated for, and at what dilution?
bsm-70369M is validated for WB (1:300-5000); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does P-Cadherin (N-terminal region) Antibody (bsm-70369M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70369M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise P-Cadherin (N-terminal region) Antibody (bsm-70369M)?
Clone (M596) was generated from a recombinant protein containing amino acids in the N-terminal region of human P-Cadherin. This sequence is highly conserved in mouse and rat P-cadherin.