Calnexin (N-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70372m |
| Product Name | Calnexin (N-terminal region) Antibody |
| Applications |
WB
|
| Specificity | This antibody detects a 90 kDa* protein corresponding to the apparent molecular mass of Calnexin on SDS-PAGE immunoblots of human HeLa and rat A7r5 cells. In immunocytochemistry, anti-Calnexin specifically stains endoplasmic reticulum and related vesicular structures in paraformaldehyde fixed and NP-40 permeabilized cells. This sequence has high homology to similar regions in rat and mouse Calnexin. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M437 was generated from a recombinant protein corresponding to amino acid residues in the N-terminal region of human calnexin. |
| Clonality | Monoclonal |
| Clone # | M437 |
| Isotype | IgG1 |
| Purification | Purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P27824 |
| Synonyms | IP90, P90 |
| Background | Calnexin is a 90 kDa integral membrane protein located primarily in the endoplasmic reticulum (ER). The structure of calnexin includes a long N-terminal calcium-binding domain that extends into the lumen of the ER and a short, acidic cytosolic domain. Calnexin associates with several cell surface proteins as they pass through the ER, and may be involved in the Ca2+-dependent retention of proteins in the ER. The amino acid sequence of calnexin is highly conserved among various species and is similar in sequence to calreticulin, another Ca2+-binding protein found in the ER. Phosphorylation may regulate the activity of the C-terminal region of Calnexin. Both proline-dependent kinase and casein kinase sites have been identified, and the phosphorylation of these sites may regulate calnexin functions involved with detection of ER protein quality control and transport. |
| Application Dilution | |
| WB | |