δ1-Catenin (central region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70388M-100uL |
| Product Name | δ1-Catenin (central region) Antibody |
| Applications |
WB
IP
|
| Specificity | The antibody detects a 110 kDa* protein corresponding to the molecular mass of δ1-Catenin on SDS-PAGE immunoblots of human A431 and HUVEC cells. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M354) was generated from a peptide that includes amino acids from the central region of human δ1-Catenin. This peptide sequence is highly conserved in rat and mouse δ1-Catenin. |
| Clonality | Monoclonal |
| Clone # | M354 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | O60716 |
| Synonyms | pp120 Src substrate, p120 |
| Background | Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is δ1-Catenin (central region) Antibody (bsm-70388M) validated for, and at what dilution?
bsm-70388M is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does δ1-Catenin (central region) Antibody (bsm-70388M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70388M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise δ1-Catenin (central region) Antibody (bsm-70388M)?
Clone (M354) was generated from a peptide that includes amino acids from the central region of human δ1-Catenin. This peptide sequence is highly conserved in rat and mouse δ1-Catenin.