c-Cbl (C-terminal region) Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-70393m
Product Name	c-Cbl (C-terminal region) Antibody
Applications	WB
Specificity	The antibody detects a 120 kDa* protein in human Jurkat cells. This sequence is highly conserved in rat and mouse c-Cbl.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Clone (M159) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human c-Cbl.
Clonality	Monoclonal
Clone #	M159
Isotype	IgG1
Purification	Purified by Protein A.
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Swiss Prot	P22681
Synonyms	E3 ubiquitin ligase CBL, RING finger 55
Background	c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain and a C3HC4 RING finger motif. c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol 3 kinase (PI3K), Crk and 14-3-3 proteins. In human cancer tissues, c-Cbl is frequently tyrosine phosphorylated in a tumor-specific manner. Src-family kinases primarily phosphorylate c-Cbl at Tyr-700, Tyr-731, and Tyr-774. These sites of phosphorylation provide docking sites for downstream signaling components, such as Grb-2, PI3K, and Fyn.
Application Dilution
WB