CD63 (Extracellular region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70406M-100uL |
| Product Name | CD63 (Extracellular region) Antibody |
| Applications |
WB
|
| Specificity | Clone M040 mouse monoclonal antibody has high reactivity to CD63 at the plasma membrane in immunocytochemical assays, and weakly detects a 30-60 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, MeWo, and A549 cells. The antibody does not detect denatured CD63 in western blot assays. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M040) was generated from a proprietary antigen related to the native human CD63 expressed in MeWo melanoma cell line. |
| Clonality | Monoclonal |
| Clone # | M040 |
| Isotype | IgG2a |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P08962 |
| Synonyms | CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491 OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, MLA1, TSPAN30 |
| Background | Tetraspanins comprise a large superfamily of cell surface-associated membrane proteins with four transmembrane domains. On cell membranes, tetraspanins form networks of various proteins called tetraspanin-enriched microdomains (TEMs). CD63 was the first characterized tetraspanin and it is found in TEMs, as well as late endosomes and lysosomes. In late endosomes, CD63 is enriched on the intraluminal vesicles, and can be secreted as exosomes through fusion of endosomes with the plasma membrane. The complex localization pattern of CD63 suggests that its intracellular trafficking and distribution must be tightly regulated. CD63 contains N-link glycosylation sites that produce diverse CD63 molecules that range from 30 to 60 kDa depending on cell type. CD63 is an important exosomal marker in cancer cells, and may be involved in cancer progression and metastasis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CD63 (Extracellular region) Antibody (bsm-70406M) validated for, and at what dilution?
bsm-70406M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD63 (Extracellular region) Antibody (bsm-70406M) react with?
Tested reactivity: Human.
How should bsm-70406M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise CD63 (Extracellular region) Antibody (bsm-70406M)?
Clone (M040) was generated from a proprietary antigen related to the native human CD63 expressed in MeWo melanoma cell line.