CD147/Emmprin/Basigin (Extracellular region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70410M-100uL |
| Product Name | CD147/Emmprin/Basigin (Extracellular region) Antibody |
| Applications |
WB
|
| Specificity | Clone M052 mouse monoclonal antibody detects a 38-58 kDa* protein on SDS-PAGE immunoblots of human SK-MES1, MDA-MB-231, MeWo, A431, LNCaP, and NCI-H1915 cells, as well as human brain tissues. The antibody works for western blot and ELISA detection of CD147. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M052) was generated from a proprietary antigen related to the native human CD147 expressed in NCI-H1915 lung cancer cell line. |
| Clonality | Monoclonal |
| Clone # | M052 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | P35613 |
| Synonyms | Basigin, 5F7, Collagenase stimulatory factor Extracellular matrix metalloproteinase,EMMPRIN, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, TCSF, CD147, BSG |
| Background | D147 (Basigin/EMMPRIN) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. CD147 is a glycosylated protein with four known isoforms with isoform 2 being the most abundantly expressed. In many cancers, CD147 is overexpressed and stimulates the secretion of extracellular matrix metalloproteinases by tumor fibroblasts, which can promote tumor progression. CD147 is co-expressed with the amino acid transporter, CD98h, in metabolically active cells such as lymphocytes, macrophages, and tumor cells. CD147 interaction with caveolin and cyclophilins regulates its functions, and this protein may function outside of tumor cells through cell shedding and microvesicle release. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CD147/Emmprin/Basigin (Extracellular region) Antibody (bsm-70410M) validated for, and at what dilution?
bsm-70410M is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD147/Emmprin/Basigin (Extracellular region) Antibody (bsm-70410M) react with?
Tested reactivity: Human.
How should bsm-70410M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise CD147/Emmprin/Basigin (Extracellular region) Antibody (bsm-70410M)?
Clone (M052) was generated from a proprietary antigen related to the native human CD147 expressed in NCI-H1915 lung cancer cell line.