CD244/2B4/SLAMF4 (Extracellular region) M053 Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70412M |
| Product Name | CD244/2B4/SLAMF4 (Extracellular region) M053 Antibody |
| Applications |
WB
IP
|
| Specificity | Clone M053 mouse monoclonal antibody detects a 110 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human THP1 monocytes. The antibody prefers the native CD244 protein, but also detects denatured CD244. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M053) was generated from a recombinant protein that included the extracellular region of human CD244 protein. |
| Clonality | Monoclonal |
| Clone # | M053 |
| Isotype | IgG1 |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q9BZW8 |
| Synonyms | Natural killer cell receptor 2B4, 2B4, NK cell activation-inducing ligand, NAIL NK cell type I receptor protein 2B4, NKR2B4, h2B4, SLAM family member 4, SLAMF4, Signaling lymphocytic activation molecule 4, CD244 |
| Background | CD244 (Natural killer (NK) cell receptor 2B4/SLAMF4) is an Ig superfamily signaling lymphocyte activation molecule (SLAM) receptor. Like all SLAM family receptors, it has an extracellular segment with two immunoglobulin (Ig)-like domains, and a cytoplasmic domain containing four immunoreceptor tyrosine-based switch motifs. CD244 does not act as a selfligand similar to other SLAM family receptors. It binds CD48, a transmembrane receptor ubiquitously expressed on hematopoietic cells. CD244 activity is controlled by the presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Downstream signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Activation of CD244 stimulates NK cell cytotoxicity, production of IFN-γ and granule exocytosis. CD244 is involved in the regulation of CD8+ T-cell proliferation, and inhibits inflammatory responses in dendritic cells (DCs). In cancers, CD244 shows increased expression in intratumoral DCs and myeloid suppressor cells, and anti-CD244 therapies may increase infiltrating T-cells and impair tumor growth. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is CD244/2B4/SLAMF4 (Extracellular region) M053 Antibody (bsm-70412M) validated for, and at what dilution?
bsm-70412M is validated for WB (1:300-5000); IP (1-2ug); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD244/2B4/SLAMF4 (Extracellular region) M053 Antibody (bsm-70412M) react with?
Tested reactivity: Human.
How should bsm-70412M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise CD244/2B4/SLAMF4 (Extracellular region) M053 Antibody (bsm-70412M)?
Clone (M053) was generated from a recombinant protein that included the extracellular region of human CD244 protein.