EB1/EB2/EB3 (C-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70458M |
| Product Name | EB1/EB2/EB3 (C-terminal region) Antibody |
| Applications |
WB
|
| Specificity | The antibody detects a 30 kDa* protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB1 by immunocytochemistry in HeLa and A431 cells. The antibody may weakly detect endogenous EB2 and EB3 in some cells and tissues. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone KT65 was generated from a GST fusion protein containing amino acids from the C-terminal region mouse EB1. This sequence is highly conserved in human and rat EB1. |
| Clonality | Monoclonal |
| Clone # | KT65 |
| Isotype | IgG2a |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 0.05% NaN3 |
| Storage Condition | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy |
| Target | |
| Swiss Prot | Q61166 |
| Synonyms | Microtubule RP/EB, MAPRE1, APC, EB1, EB2, EB3 End-binding |
| Background | The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (α) and short (β) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP). The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is EB1/EB2/EB3 (C-terminal region) Antibody (bsm-70458M) validated for, and at what dilution?
bsm-70458M is validated for WB (1:300-5000); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EB1/EB2/EB3 (C-terminal region) Antibody (bsm-70458M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70458M be stored?
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cy Storage buffer: PBS + 0.05% NaN3
What immunogen was used to raise EB1/EB2/EB3 (C-terminal region) Antibody (bsm-70458M)?
Clone KT65 was generated from a GST fusion protein containing amino acids from the C-terminal region mouse EB1. This sequence is highly conserved in human and rat EB1.