| Overview |
| bsm-70511m |
| GSK-3_ (N-terminal region) Antibody |
| WB, IHC |
| The antibody detects a 46 kDa* protein corresponding to the apparent molecular mass of GSK-3_ on SDS-PAGE immunoblots of rabbit fibroblasts, mouse brain, as well as human SKN-SH and A431 cells. This sequence is highly conserved in rat and mouse GSK-3_, and has 65% homology to similar regions in GSK-3_. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Mouse |
| Clone M131 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human GSK-3_. |
| Monoclonal |
| M131 |
| IgG1 |
| Purified by Protein A. |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target |
| P49841 |
| Glycogen synthase kinase beta3 |
| Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (_ and _) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3_ plays a critical role in cell survival by phosphorylating nuclear factor-_B (NF-_B) p65 subunit, leading to NF-_B transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3_), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of _-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3_ activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3_ (Serine 21 in GSK-3_). This phosphorylation may be involved in later phases of neuronal apoptosis. |
| Application Dilution |
| WB |
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| IHC |
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