GSK-3_/_ (Tyr-279/Tyr-216), Phosphospecific Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-70512m
Product Name	GSK-3_/_ (Tyr-279/Tyr-216), Phosphospecific Antibody
Applications	WB
Specificity	The antibody detects 46/50 kDa* proteins corresponding to the apparent molecular mass of GSK-3_ and GSK-3_ on SDS-PAGE immunoblots of pervanadate treated rabbit fibroblasts, as well as treated human SKN-SH and A431 cells. This peptide sequence is also found in GSK-3_ (Tyr-279) and is highly conserved in GSK-3 genes in rat and mouse.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Clone M132 was generated from a phospho-GSK-3_ (Tyr-216) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 216 of human GSK-3_.
Modification Site	Tyr-216
Clonality	Monoclonal
Clone #	M132
Isotype	IgG1
Purification	Purified by Protein A.
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Swiss Prot	P49840
Synonyms	Glycogen synthase kinase beta3
Background	Glycogen synthase kinase-3 (GSK-3) has been implicated in fundamental cell processes such as cell fate determination, metabolism, transcriptional control, and oncogenesis. Two GSK-3 genes (_ and _) have been cloned in mammals and these kinase homologues show strong sequence conservation within their catalytic domain. GSK-3_ plays a critical role in cell survival by phosphorylating nuclear factor-_B (NF-_B) p65 subunit, leading to NF-_B transactivation in hepatocytes. Phosphorylation regulates the activity of both GSK-3 genes. MEK1/2 can phosphorylate tyrosine 216 (tyrosine 279 in GSK-3_), which stimulates GSK-3 kinase activity. Tyr-216 phosphorylation is required for GSK-mediated down-regulation of _-catenin activity. Also, TRAIL stimulation can increase Tyr-216 phosphorylation, and GSK-3_ activity may suppress TRAIL-induced apoptosis. Inactiviation of GSK-3 occurs through Akt phosphorylation of serine 9 of GSK-3_ (Serine 21 in GSK-3_). This phosphorylation may be involved in later phases of neuronal apoptosis.
Application Dilution
WB