His (C-terminal) Tag Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70513M-100uL |
| Product Name | His (C-terminal) Tag Antibody |
| Applications |
WB
IP
IHC
FCM
|
| Specificity | Clone M050 detects recombinant proteins with C-terminal His-tag epitope. The antibody binds 6 and 10 His-tag epitopes on the C-terminal side of a protein, but does not bind N-terminal His-tag epitopes. The antibody works for western blot, immunoprecipitation, immunocytochemistry, flow cytometry, and ELISA capture. |
| Reactivity | Mouse, Rat, Bacteria, Tags |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone (M050) was generated from a recombinant protein containing a C-terminal His-tag. |
| Clonality | Monoclonal |
| Clone # | M050 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | N/A |
| Synonyms | His Tag, 6x His Tag, HHHHHH |
| Background | Epitope tagging (e.g. His-tag) is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). A variety of plasmids contain DNA that encodes an amino- or carboxy-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. These plasmids facilitate expression of His-tagged recombinant proteins that can be isolated or purified by immobilized metal affinity chromatography. Anti-His Tag antibodies can be used to detect recombinant proteins with the His-Tag in many different immunoassays, including western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, flow cytometry, ELISA, and chromatin immunoprecipitation assays. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
| IHC | 1:50-200 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is His (C-terminal) Tag Antibody (bsm-70513M) validated for, and at what dilution?
bsm-70513M is validated for WB (1:300-5000); IP (1-2ug); IHC (1:50-200); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does His (C-terminal) Tag Antibody (bsm-70513M) react with?
Tested reactivity: Mouse, Rat, Bacteria, Tags.
How should bsm-70513M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise His (C-terminal) Tag Antibody (bsm-70513M)?
Clone (M050) was generated from a recombinant protein containing a C-terminal His-tag.