I_B_ (Ser-32/Ser-36), Phosphospecific Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-70521m
Product Name	I_B_ (Ser-32/Ser-36), Phosphospecific Antibody
Applications	WB, IP
Specificity	The antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of Jurkat cells treated with calpain inhibitor (ALLN) followed by TNF_, but the antibody does not detect this band in untreated cells. This peptide sequence is highly conserved in mouse, rat, dog, cow, and pig I_B_.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Clone 39A1413 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 32 and 36 of human I_B_.
Modification Site	Ser-32/Ser-36
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Purification	Purified by Protein A.
Storage Buffer	PBS + 0.5% BSA and 0.05% NaN3
Storage Condition	Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20 C or -80 C, while keeping a working aliquot stored at 4 C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Target
Swiss Prot	P25963
Synonyms	IkB, MAD3, IkappaBalpha, NFkappaB inhibitor IkBa
Background	The NF-_B/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory I_B proteins. Activation of I_B_ occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-_B. This pathway of I_B_ regulation occurs in response to various NF-_B-activating agents, such as TNF_, interleukins, LPS, and irradiation. An alternative pathway for I_B_ regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of I_B_ and NF-_B-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in I_B_ nuclear translocation and inhibition of NF-_B-activation. Thus, tyrosine phosphorylation of I_B_ may be an important regulatory mechanism in NF-_B signaling.
Application Dilution
WB
IP