Mitofilin Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70546M-100uL |
| Product Name | Mitofilin Antibody |
| Applications |
WB
IP
|
| Specificity | Clone M027 detects a 90 kDa* band corresponding to the molecular mass of mitofilin on SDS-PAGE immunoblots of native or denatured human PC3, MDA-MB-231, A431, A549, and MeWo cell lysates, as well as human breast, skin, and brain tissues. |
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line. |
| Clonality | Monoclonal |
| Clone # | M027 |
| Isotype | IgG1 |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q16891 |
| Synonyms | MICOS complex subunit MIC60, Cell proliferation-inducing gene 4/52 protein,Mitochondrial inner membrane protein Mitofilin p87/89, IMMT, HMP, MIC60, MINOS2, PIG4, PIG52, MINOS |
| Background | Mitofilin is an important organizer of mitochondrial architecture. The mitofilin sequence encodes a polypeptide with a central alpha-helical region with predicted coiled coil domains flanked by globular amino and carboxy termini. There are four isoforms of mitofilin, and 90/91 kDa mitofilin forms have been observed in western blots. Mitofilin is located in the inner mitochondrial membrane and interacts with several protein complexes of the outer membrane, thereby generating contact sites between the two membrane systems of mitochondria. These mitofilin-containing hetero-oligomeric protein complexes form the mitochondrial inner membrane organizing system (MINOS). MINOS integrity is required for the structural maintenance of the inner mitochondrial membrane. This mitochondrial region contains cristae membranes that form large tubular invaginations that protrude into the mitochondrial matrix. These cristae membranes contain the enzyme complexes of the oxidative phosphorylation machinery. MINOS deficiency causes loss of crista junction structures and the detachment of cristae from the inner boundary membrane. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is Mitofilin Antibody (bsm-70546M) validated for, and at what dilution?
bsm-70546M is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mitofilin Antibody (bsm-70546M) react with?
Tested reactivity: Human.
How should bsm-70546M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Mitofilin Antibody (bsm-70546M)?
Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line.