NLRC4 (Ser-533), Phosphospecific Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-70567t
Product Name	NLRC4 (Ser-533), Phosphospecific Antibody
Applications	WB
Specificity	This antibody antibody detects a 110 kDa* protein on SDS-PAGE immunoblots of PMA-differentiated THP1 human macrophages. In addition, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) peptide, but does not detect this peptide after lambda phosphatase treatment. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rat
Source	Clone M549 was generated from a phospho-NLRC4 (Ser-533) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding serine 533 in mouse NLRC4.
Modification Site	Ser-533
Clonality	Monoclonal
Clone #	M549
Isotype	N.T.
Purification	Antigen Affinity purification
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Swiss Prot	Q3UP24
Synonyms	CARD12, CLAN1, IPAF, NLR family CARD domain-containing protein 4, NOD-like receptor 4
Background	The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family is a diverse family of cytoplasmic innate immune receptors that are involved in recognition of pathogen-associated molecular patterns. NLRs are important for pathogen sensing, transcriptional activation of proinflammatory cytokines and activation of inflammatory caspases. NLRC4 (IPAF, CARD12) forms the inflammasome that responds to bacterial flagellin. This inflammasome is activated by NLRC4 oligomerization, NAIP protein binding, and activation of caspase-1 leading to pyroptosis. NLRC4 is phosphorylated on Ser-533 by PKC_ following infection of macrophages with S. typhimurium. Mutant forms of NLRC4 demonstrate that an unphosphorylatable form (S533A) does not activate caspase-1 and pyroptosis in response to S. typhimurium, while a phosphomimetic NLRC4 (S533D) mutant causes rapid macrophage pyroptosis without infection. Thus, PKC_ phosphorylation of NLRC4 (S533) may be a critical event in inflammasome activation and host innate immunity.
Application Dilution
WB