p38_ MAP Kinase (C-terminal) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70584M |
| Product Name | p38_ MAP Kinase (C-terminal) Antibody |
| Applications |
WB
|
| Specificity | This antibody detects a 38 kDa* protein corresponding to the apparent molecular mass of p38α on SDS-PAGE immunoblots of human A431 and rat A7r5 cells. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M548 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human p38α. This sequence is identical in rat and mouse p38α. |
| Clonality | Monoclonal |
| Clone # | M548 |
| Isotype | IgG1 |
| Purification | Purified by Protein G. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q16539 |
| Synonyms | MAPK, p38, p38alpha, p38MAPK |
| Background | p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (α,β,γ,δ) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternative pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is p38_ MAP Kinase (C-terminal) Antibody (bsm-70584M) validated for, and at what dilution?
bsm-70584M is validated for WB (1:300-5000); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p38_ MAP Kinase (C-terminal) Antibody (bsm-70584M) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-70584M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise p38_ MAP Kinase (C-terminal) Antibody (bsm-70584M)?
Clone M548 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human p38α. This sequence is identical in rat and mouse p38α.