p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-70585m
Product Name	p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody
Applications	WB
Specificity	This antibody detects a 38 kDa* protein corresponding to the apparent molecular mass of p38_ on SDS-PAGE immunoblots of pervanadate treated human Jurkat and A431 cells, as well as anisomycin treated human HeLa cells. This peptide sequence is highly conserved in the p38_,g, and d MAPKs, and is identical in human and mouse p38_.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Clone M139 was generated from a phospho-p38_ (Thr-180/Tyr-182) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 180 and tyrosine 182 of rat p38_.
Clonality	Monoclonal
Clone #	M139
Isotype	IgG1
Purification	Purified by Protein A.
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Swiss Prot	P70618
Synonyms	MAPK, p38, p38alpha, p38MAPK
Background	p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase. This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Four isoforms of p38 MAPK (_,_,_,_) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growth factors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. T cells possess an alternative pathway for p38 activation where stimulation of the antigen receptor (TCR) induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 and catalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.
Application Dilution
WB