p130 Cas (C-terminal region) Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70587m |
| Product Name | p130 Cas (C-terminal region) Antibody |
| Applications |
WB
IP
IHC
|
| Specificity | The antibody detects a 130 kDa* protein corresponding to the molecular mass of p130 Cas on SDS-PAGE immunoblots of human A431, endothelial, and Hct116 cells. This rat sequence is highly conserved in human, mouse, and chicken p130 Cas. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M144 was generated from a recombinant protein containing amino acid residues in the C-terminal region of rat p130 Cas. |
| Clonality | Monoclonal |
| Clone # | M144 |
| Isotype | IgG1 |
| Purification | Purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | Q63767 |
| Synonyms | BCAR1, Cas |
| Background | p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking protein containing multiple protein-protein interaction domains. The N-terminal SH3 domain functions as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with tyrosine kinases and phosphatases. The C-terminal Src binding domain contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs). Phosphorylation of this domain at Tyr-762 in rat (Tyr-668 in mouse) promotes this interaction. The p130 Cas central substrate domain is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, and Tyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site can attenuate caspase cleavage, while dephosphorylation occurs during apoptosis and may facilitate p130 Cas degradation. |
| Application Dilution | |
| WB | |
| IP | |
| IHC | |