Phosphotyrosine Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-70608M-100uL |
| Product Name | Phosphotyrosine Antibody |
| Applications |
WB
IP
|
| Specificity | This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells. |
| Reactivity | Human, Mouse, Rat, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells. |
| Clonality | Monoclonal |
| Clone # | M375 |
| Isotype | IgG2b |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target | |
| Swiss Prot | N/A |
| Synonyms | Phosphotyrosine mAb |
| Background | Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is Phosphotyrosine Antibody (bsm-70608M) validated for, and at what dilution?
bsm-70608M is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phosphotyrosine Antibody (bsm-70608M) react with?
Tested reactivity: Human, Mouse, Rat, Chicken.
How should bsm-70608M be stored?
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. Storage buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
What immunogen was used to raise Phosphotyrosine Antibody (bsm-70608M)?
Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.