| Overview |
| bsm-70615m |
| PKC_ (N-terminal region) Antibody |
| WB |
| This antibody detects a 79 kDa* protein corresponding to the molecular mass of PKC_ on SDS-PAGE immunoblots of human Jurkat cell lysates. This sequence is conserved in human and rat PKC_, and has low homology to other PKC family members. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Mouse |
| Clone (M217) was generated from a recombinant mouse PKC_ protein that included amino acids residues in the N-terminal region. |
| Monoclonal |
| M217 |
| IgG2a |
| Purified by Protein A. |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target |
| A6H667 |
| PKC theta |
| The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (_, _1, _II, and _) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. The phosphorylation of multiple sites in PKCs regulates their activity |
| Application Dilution |
| WB |
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